DNA / RNA Sequencing

DNA & RNA Sequencing Services

DNA and RNA sequencing plays a crucial role in biologics, gene therapy, and biosimilar development, enabling researchers to answer a wide range of quality control, process, and development questions. From integration site analysis of gene therapies to the validation of vector genome integrity and monoclonality, sequencing technologies provide essential insights into genome structure, integrity, and stability at every stage of biologics production.

At Biofidus, we provide accurate and reliable nucleic acid analysis using next-generation sequencing (NGS) with Oxford Nanopore Technologies (ONT). This long-read sequencing technology is ideal for addressing complex challenges.

The high-throughput, fast turnaround times, and high-quality data from ONT sequencing allow us to meet the needs of biotech and pharmaceutical companies, supporting the entire drug development cycle, from early discovery through clinical development.

Our team of experts is ready to provide tailored sequencing solutions for your specific needs, ensuring that you get the most accurate, reliable, and actionable data for your biologic or gene therapy project. In line with your needs, we adapt our platform methods to your product, its formulation matrix, and your distinct analytical questions.

Get in contact with us to quickly find the optimal setup for your analytical challenge and to speak directly from expert to expert.

Our Capabilities

  • TCR sequencing
  • 16S sequencing
  • Long read sequencing (ONT)​
  • Genome integrity analysis (truncations, duplications, fusions, etc.)​
  • Amplicon and vector/plasmid characterization​
  • Analysis of repetitive genetic elements​
  • poly(A) characterization
  • Qualitative and quantitative analysis of process-related DNA contaminations​

Just what you need? Contact us!

FAQ

Find answers to frequently asked questions about our analytical services, methods, technologies, and studies, from molecular characterization and bioactivity to comparability, stability, and fit-for-purpose method development. 

Yes. We combine sequencing and qPCR where appropriate to address copy number and quantitative readouts alongside qualitative integrity assessment.

Yes. Our sequencing workflows support integration site analysis, detection of truncations, duplications, and fusions to verify genome integrity.

Yes. We provide DNA/RNA sequencing services using Oxford Nanopore Technologies (ONT) to address genome integrity, sequence confirmation and QC questions across development.

Yes. We can detect and quantify process-related DNA contaminations and support impurity questions in the context of broader CMC analytics.

Yes. At Biofidus, we use ONT long-read sequencing to support amplicon and vector/plasmid characterization as well as the structural analysis of repetitive genetic elements. This helps confirm construct integrity and resolve complex sequence regions that are highly relevant for development and quality control.

Oligonucleotide services

Advancing research through tailored bioanalytical solutions.

  • Gene integration site analysis in cell lines or genetically modified organisms
  • Validation of genome integrity of gene therapy vectors
  • Monoclonality assessment to ensure homogeneous cell populations
  • Gene copy number determination
  • Structural analysis of tandem repeats and repetitive sequences
  • Transcriptomics and expression profiling
  • Detection and quantification of host cell DNA impurities

TECHNOLOGIES USED

  1. Oxford Nanopore Technologies sequencing (ONT)
  2. Quantitative PCR (qPCR)

Tell us about Your Project

We‘ll advise you to define your assay needs.

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Our Expert

Across end-to-end delivery, our experts remain on hand with deep scientific expertise whenever needed, providing oversight to streamline and de-risk milestone delivery.
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