Metabolites

Metabolite Analysis for Biopharmaceutical Processes

Metabolites are small intermediary molecules formed in biochemical pathways and are central to cell signalling, regulation of cellular functions and energy production. In biopharmaceutical manufacturing, targeted metabolite analysis provides key insights into cell culture conditions, such as nutrient status, oxygen supply and overall metabolic state, enabling you to improve cell growth, productivity and product quality.

Beyond real-time assessment of cell growth and culture performance, metabolite profiling can also reveal process-related impurities, including microbiological contaminants and unwanted by-products, making it a powerful tool for process monitoring, troubleshooting and quality control.

At Biofidus, we offer standardized chromatographic assays for a broad range of metabolites, including amino acids, organic acids, polyamines, surfactants, sugars, fatty acids, vitamins, nucleotides, secondary messengers and process-related impurities.

In line with your needs, we adapt and, if required, qualify our platform methods to address your specific process, matrix and analytical questions.

Get in contact with our expert to find the optimal strategy for your project and to speak directly from scientist to scientist.

Our Capabilities

  • Quantification and identification of metabolites and impurities
  • Assessment of source and impact on safety of detected metabolites
  • Guidance on process optimization
  • Method qualification
  • Stability studies
  • Forced degradation studies

Just what you need? Contact us!

FAQ

Find answers to frequently asked questions about our analytical services, methods, technologies, and studies, from molecular characterization and bioactivity to comparability, stability, and fit-for-purpose method development. 

Yes. Metabolite profiling can reveal unwanted by-products and indicators of process deviations or microbiological contamination.

Yes. We provide standardized chromatographic metabolite panels and tailor scope to your process monitoring and troubleshooting needs.

Yes. Metabolite profiling can reveal unwanted by-products and abnormal metabolic patterns that may indicate process deviations, nutrient imbalance, or microbiological contamination. At Biofidus, we use targeted metabolite analysis to support troubleshooting, process understanding, and the optimization of cell culture performance.

We can work with cell culture media, feeds and process intermediates; we’ll advise on sample handling, storage and required volumes for your target panel.

Key metabolites include amino acids, organic acids and sugars that reflect nutrient status, oxygen supply and metabolic state and can impact productivity and quality.

Analytical techniques

Advancing research through tailored bioanalytical solutions.

  • LC-UV
  • LC-PAD
  • LC-FLD
  • LC-QQQ-MS
  • LC-QTOF-MS
  • GC-MS on request

Tell us about Your Project

We‘ll advise you to define your assay needs.

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Our Expert

Across end-to-end delivery, our experts remain on hand with deep scientific expertise whenever needed, providing oversight to streamline and de-risk milestone delivery.
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